MB119-B: 2x hot-start high-fidelity PCR Master Mix, with blue dye
* Simple operation: The PCR master mix simplifies the assembly of PCR reaction and offers advantages of time savings, convenience, consistency, and minimal risk of contamination and pipetting errors. The tracking dye and precipitant have been added into the master mix so that the PCR product can be directly loaded for electrophoresis.
* Ultra-high fidelity: The fidelity is 53 times that of Taq DNA Polymerase and 6 times that of Pfu DNA Polymerase. For every 300,000 bases amplified, there are fewer than 5 mismatches.
* Fast amplification: normally 4-5 sec/kb; ~0.5 sec/kb if the amplification length is <1 kb; 4-150 times that of conventional PCR master mix.
* Long fragment amplification: up to 40 kb from simple templates such as λDNA and plasmids, up to 20 kb from complex templates such as genomic DNA, and up to 10 kb from cDNA templates.
* Wide adaptability: It is suitable for the amplification of various GC content fragments, has super tolerance to PCR inhibitors, and can be used for direct PCR of a variety of samples.
* Features of the hot-start high fidelity DNA polymerase (catalog No. MB118) contained in the 2x hot-start high-fidelity PCR master mix.MB040-HY2: 2x HY PCR Master Mix, with blue dye
* The PCR master mix simplifies the assembly of PCR reaction and offers advantages of time savings, convenience, consistency, and minimal risk of contamination and pipetting errors.
* The high yield PCR Master Mix has increased amplification robustness, fidelity, yield, and fragment length, as well as the ability to handle difficult or “dirty” templates.
* The tracking dye and precipitant have been added into the PCR PreMix so that the PCR product can be directly loaded for electrophoresis.
Features of the blend of Taq enzyme and a proofreading enzyme contained in the high yield PCR master mix:
* Robust processivity. Up to 10 kb human genomic and 15 kb lamda DNA fragments have been tested for amplification.
* High purity. >98% homogeneous of the Taq DNA polymerase by SDS gel electrophoresis. No contamination detected in standard PCR test reactions.
* Reproducible results. No visible activity change after storage of the Taq DNA polymerase at room temperature for 3 months to amplify a single-copy gene from human genome with high efficiency.
* Proofreading with 5′ exonuclease activity.MB067-EQ2G / MB067-EQ2B: 2x PCR PreMix, with dye (green, or blue)
* The PCR master mix simplifies the assembly of PCR reaction and offers advantages of time savings, convenience, consistency, and minimal risk of contamination and pipetting errors.
* The tracking dye and precipitant have been added into the PreMix so that the PCR product can be directly loaded for electrophoresis.
Features of the Taq DNA polymerases contained in the PCR master mix:
* High efficiency. The extension time of the Taq DNA polymerase is shorter than 30 seconds per kb DNA. Easily amplify DNA fragments up to 5 kb.
* High purity. >98% homogeneous of the Taq DNA polymerase by SDS gel electrophoresis. No contamination detected in standard PCR test reactions.
* Reproducible results. No visible activity change after storage of the Taq DNA polymerase at room temperature for 3 months to amplify a single-copy gene from human genome with high efficiency.
* Terminal transferase activity of adding a single nucleotide (adenosine) at 3'-end of the extension product, facilitating TA cloning of PCR products.